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KMID : 1161520100140040275
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Animal Cells and Systems
2010 Volume.14 No. 4 p.275 ~ p.282
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Chunghyul-dan acts as an anti-inflammatory agent in endothelial cells by regulating gene expression
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Jung Woo-Sang
Cho Jin-Gu
In Kyung-Min
Kim Jong-Min
Cho Ki-Ho
Park Jung-Mi
Moon Sang-Kwan
Kim Kyung-Wook
Park Seong-Uk
Pyee Jae-Ho
Park Sang-Gyu
Jeong Yoon-Hwa
Park Heon-Yong
Ko Chang-Nam
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Abstract
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Chunghyul-dan (CHD) is a combinatorial drug known to exert anti-inflammatory effects in endothelial cells. In this study, we employed global transcriptional profiling using cDNA microarrays to identify molecular mechanisms responsible for the anti-inflammatory activity of CHD in endothelial cells. An analysis of the microarray data revealed that transcript levels of monocyte chemotactic protein-1 (MCP-1), vascular cell-adhesion molecule-1 (VCAM-1) and activated leukocyte cell-adhesion molecule were dramatically altered in CHD-treated endothelial cells. These changes in gene expression were confirmed by RT-PCR, Western blotting and ELISA. Chronic CHD treatment also appeared to decrease MCP-1 secretion, probably as a result of decreased MCP-1 expression. In addition, we determined that chronic CHD treatment inhibited lipopolysaccharide-stimulated adhesion of THP-1 leukocytes to endothelial cells. The inhibitory effect of CHD on LPS-stimulated adhesion resulted from down-regulation of VCAM-1 expression. Transmigration of THP-1 leukocytes through endothelial cells was also inhibited by chronic CHD treatment. In conclusion, CHD controls a variety of inflammatory activities by regulating MCP-1 and VCAM-1 gene expression.
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KEYWORD
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chunghyul-dan, chemotaxis, cell adhesion, endothelial cells, transmigration
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